Achievements and associated publications

The work driven on the axis 2 involve, since the beginning of 2012, 9 teams in the 3 partner institutes. The main progresses are the following ones (selection of publications):

Cartography of the chloroplast: purification of chloroplast sub-compartments and targeted proteomic analyses

  • Ferro et al., Mol Cell Proteomics 2010;
  • Joyard et al., Prog Lipid Res 2010;
  • Rolland et al., Annu Rev Genetics 2012;
  • Bruley et al., Front Plant Sci 2012;
  • Tomizioli et al., Mol Cell Proteomics 2014

Analysis of the molecular mechanisms involved in the adaptation of the chloroplast to changing environmental conditions

  • Allorent et al., Plant Cell 2013;
  • Albrecht-Borth et al., Plant Physiol 2013;
  • Simionato et al., Eukaryot Cell 2013;
  • Allorent et al., Plant Mol Biol 2013;
  • Gläßer et al., Mol Plant 2014;
  • Nagy et al., PNAS USA 2014

Prediction of protein targeting to the chloroplast

  • Tardif et al., Mol Biol Evol 2012

Functional and structural characterization of ions and metabolites chloroplast transporters

  • Catty et al., J Biol Chem 2011;
  • Deniaud et al., PLoS One 2012;
  • Checchetto et al., PNAS USA 2012;
  • Carraretto et al., Science 2013;
  • Boutigny et al., J Exp Bot 2014

Identification of new targets of post-translational modifications (methylation) in the chloroplast

  • Mininno et al., J Biol Chem. 2012;
  • Alban et al., PLoS One 2014

Identification of chloroplast proteins interacting with calmodulin

  • Dell’Aglio et al., Mol Biosyst 2013

Structure / function analysis of chloroplasts proteins

  • Robin et al., J Mol Biol. 2010;
  • Raspail et al., J Biol Chem. 2011;
  • Graindorge et al., J Biol Chem 2014

Analysis of the compartmentation and transport of lipids between the chloroplast and other compartments of the cell

  • Botella C et al., Importance of phosphatidylcholine on the chloroplast surface. Progress in Lipid Research, 2017, 65: 12-23
  • Degraeve-Guilbault Cet al., Glycerolipidome peculiarities of the smallest green picoeukaryote Ostreococcus tauri. Plant Physiology, 2017, 173(4): 2060-2080
  • Dolch LJ et al., A palmitic acid elongase affects eicosapentaenoic acid and plastidial monogalactosyldiacylglycerol levels in Nannochloropsis. Plant Physiology, 2017, 173(1): 742-759
  • Manduc M et al., Tight cohesion between glycolipid membranes results from balanced water-headgroup interactions. Nature Communications, 2017, 8:14899
  • Meï CE et al., C1 metabolism inhibition and nitrogen deprivation trigger triacylglycerol accumulation in Arabidopsis thaliana cell cultures and highlight a role of NPC in phosphatidylcholine-to-triacylglycerol pathway. Frontiers in Plant Science, 2017
  • Michaud M, Prinz WA and Jouhet J. Glycerolipid synthesis and lipid trafficking in plant mitochondria. FEBS Journal, 2017, 284(3): 376-390
  • Alboresi A et al., Light remodels lipid biosynthesis in Nannochloropsis gaditana by modulating carbon partitioning between organelles. Plant Physiology, 2016, 171(4): 2468-2482
  • Bastien O et al., New insights on thylakoid biogenesis in plant cells. Chatper 1. In: International review of cell and molecular biology. Edited by Kwang W. Jeon, 2016, 323: 1-30, pp 286
  • Botella C et al., ALA10, a phospholipid flippase, controls FAD2/FAD3 desaturation of phosphatidylcholine in the ER, and affects chloroplast lipid composition in Arabidopsis thaliana. Plant Physiology, 2016, 170(3): 1300-1314
  • Chevalier F et al., Cuyàs Carrera L, Nussaume L and Maréchal E, Chemical genetics in dissecting membrane glycerolipid functions. In: Lipids in Plant and Algae Development (Y. Nakamura and Y. Li-Beisson, Eds), Springer, 2016, 159-175
  • Flori S et al., Ultrastructure of the periplastidial compartment of the diatom Phaeodactylum tricornutum. Protist, 2016, 167(3): 254–267*
  • Michaud M et al., AtMic60 is involved in plant mitochondria lipid trafficking and is part of a large complex. Current Biology, 2016, 26(5): 627-639
  • Rivasseau C et al., Coccomyxa actinabiotis sp. nov. (Trebouxiophyceae, Chlorophyta), a new green microalga living in the spent fuel cooling pool of a nuclear reactor. Journal of Phycology, 2016, 52: 689-703
  • Rocha J et al., Structural insights and membrane binding properties of MGD1, the major galactolipid synthase in plants. Plant Journal, 2016, 85(5): 622-633
  • Stopka SA et al.,  Turnover rates in microorganisms by laser ablation electrospray ionization mass spectrometry and pulse-chase analysis. Analytica Chimica Acta, 2016, 902: 1-7

Initiation of the modeling of the metabolism (ChloroKB database) and the main functions of the chloroplast

  • Gloaguen P,  Curien G et al.,  ChloroKB:a web-application for the integration of knowledge related to chloroplast metabolic network. Plant Physiol. 2017 Apr 25. pii: pp.00242.2017
  • Curien et al., Methods Mol Biol 2014;
  • Ebenhöh et al., Philos Trans R Soc Lond B Biol Sci 2014

Link to the website :


Teams involved in the project

1. BIG


  • Team 1 – team leader Norbert Rolland, DR1 CNRS
  • Team 2 – team leader Eric Maréchal DR1 CNRS
  • Team 4 – team leader  Stéphane Ravanel, DR2 INRA
  • Team 7 – team leader Thomas Pfannschmidt (Prof UJF) / Livia Merendino (CR1 CNRS) organization of the “Chloroplast” workshop end of 2014


  • Team EDyP – team leader Virginie Brun (DR CEA)


 2. IBS